Test Description:
Various cytogenetic abnormalities have been observed in association with ALL including translocation 12;21, 11q23 rearrangements, 19p13.3 rearrangements, addition of chromosomes 4, 5, 10 and 17 as well as translocation 9;22. Our panel screens for these common cytogenetic abnormalities. A chromosome analysis may be requested in addition to the ALL panel.
| t(12;21) | ES (Extra Signal) | TEL(ETV6)/AML1(RUNX1, CBFA2) (child) |
| t(0;22) | DCDF (Dual Color Dual Fusion) | BCR/ABL1 (adult) |
| 11q23 | BAP (Break Apart) | MLL (adult/child) |
| 19p13.3 | BAP | E2A(TCF3) (child) |
| +4 | SC (Single Color) | D4Z1 (child) |
| +10 | SC | D10Z1 (child) |
| +17 | SC | D17Z1 (child) |
| +5 | | if hyperdiploid, +5 will be checked for poor prognosis |
Methods:
Two hundred interphase cells are screened via FISH for multiple loci. This test was developed and its performance characteristics determined by Michigan State University Cytogenetics Laboratory as required by the CLIA 1988 regulations. Our laboratory has established and verified the test's accuracy and precision. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. However, the FDA has determined that such clearance is not necessary to use this test for clinical purposes.
Specimen Requirements:
7 ml of blood (2-3 ml of blood for infants) or 1-5 ml of bone marrow in a sodium heparinized vacutainer (green top tube). Ship bone marrow specimens immediately.
Shipping Instructions:
Ship immediately or via overnight delivery at room temperature to the MSU Cytogenetics Laboratory, B123 Life Sciences Bldg, East Lansing, MI 48824. For specimen transport, call 517-353-2032 or 1-877-TEST-DNA. For specimens obtained on the weekend, call our pager at 517-229-6130. Please leave a return phone number and our weekend staff will contact you.
Turnaround Time: 7 days
Test CPT Codes: 88237, 88271x2, 88275x2, 88291